Kekik ve mercanköşk diğer yağlara göre daha yüksek antimikrobiyal aktivite gösterirken, her iki mikroorganizmaya karşı en yüksek antimikrobiyal aktiviteyi. Pazarbasi, B., Yilmaz, M., Türkiye Kosullarinda Üretimi Yapilan Spirulina platensis’ in Çesitli Ekstraktlarinin Antimikrobiyal Aktivite Kapasitesinin Belirlenmesi. antimikrobiyal aktivite, antioksidan aktivite, fenolik madde. Özet: Bu çalışmada Türkiye’nin Doğu Akdeniz Bölgesi’nden toplanan Tornabea.
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The concentration of essential oil which had the same turbidity with negative control was determined as MIC and it was corroborated by OD measurement. In a study, how mustard essential oil affected the cell membrane of Escherichia coli O But, this does not mean that gram-positive bacteria are always more susceptible. The obtained MIC values against E. Diffusion methods can be classified as disc diffusion and agar well diffusion methods while dilution method can be classified as agar dilution and broth dilution methods.
MBC is defined as concentration where To achieve this purpose the food industry has used different physical and chemical methods which diminish microbial growth or inhibit microorganisms and prevent or delay. There is therefore still a need for new methods of reducing or eliminating food borne pathogens. Thyme and origanum essential oils showed higher antimicrobial activity than the other essential oils.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated. The obtained results are more sensitive than agar dilution method. Its effect on other antimiikrobiyal was very significant except dry herbage and oil yield.
Applying different methods to determine antimicrobial activity of antikikrobiyal essential oil cause to be obtained different results. Please use this identifier to cite or link to this item: On the contrary, no aktivihe activity was observed for basil, ginger and fennel essential oils against E.
Hydrophobicity of essential oils and their components enables them to partition in antikikrobiyal lipids of the bacterial cell membrane, disturbing the structures and rendering them more permeable.
In this study, dillweed, cardamon, basil, thyme, cumin, origanum, fennel and ginger essential oils were studied for their antimicrobial activity against Escherichia coli and Staphylococcus aureus by agar well diffusion method. MIC is defined as the lowest concentration of the essential oil which inhibits bacterial growth. Also, the extracellular ATP concentration increased and a reduction of the intracellular pH was observed in both bacteria.
Antimikrobjyal is also known that chemical composition of essential oils from a particular plant species can vary according to the geographical origin and harvesting period.
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Intracellular pH and ATP concentration and the release of cell a,tivite were measured when mustard essential oil was in contact with E. They affect microbial cells by various antimicrobial mechanisms, including attacking the phospholipid bilayer of the cell membrane and distrupting enzyme systems. However, origanum essential oil showed the highest inhibitory activity antimikrobiyap both bacterial species tested. There are also new concerns about chemical preservatives due to increasing occurrence of many respiratory illnesses.
Cumin and cardamon showed the lowest antimicrobial effect against E. Preservation of food materials from spoilage, mainly by microbial activity, during production, storage and marketing is an important issue in the food industry. MBC values of thyme and origanum essential oils against E. The phenolic components are chiefly responsible for the antibacterial properties of essential antimikroboyal.
However, the increased demand for safe and natural antimikrobiayl, without chemical preservatives, provokes many researchers to investigate the antimicrobial effects of natural compounds. Gram-negative bacteria are less sensitive to the antimicrobials because of the lipopolysaccharide outer membrane of this group, which restricts diffusion of hydrophobic compounds.
Essential oils can comprise more than sixty individual components.
Leakage of antimikfobiyal and other cell contents can then occur. Antimimrobiyal aim of this study was to determine the antimicrobial activity of some essential oils dillweed, cardamon, basil, thyme, cumin, origanum, fennel and ginger essential oils against Escherichia coli and Staphylococcus aureus and to determine the MIC and MBC values of the selected essential oils.
In this method obtained MIC values of E. The apparent antimicrobial efficacy of plant origin antimicrobials depends on factors such as the method of extracting essential oils from plant material,the part of a plant where the essential oil is extracted,type of solvent, volume of inoculum, growth phase, culture medium used, concentration angimikrobiyal essential oil, temperature, oxygen and intrinsic or extrinsic properties of the food such as pH, fat, protein, NaCl, and physical structure of food.
The MIC was determined by comparing the turbidity of the essential oil added well with the turbidity of negative control added well.
The main advantage of essential oils is antimikrrobiyal they can be used in any food and are generally recognized as safe GRAS. There is some evidence that minor components have a critical part to play in antimijrobiyal activity, possibly by producing a synergistic effect between other components.
However, most studies investigating the action of whole essential oils against food spoilage organisms and food borne pathogens agree that, generally, essential oils are slightly more active against gram-positive than gram-negative bacteria.
Electronic microscopy observations showed that the cell membranes of both bacteria were apparently damaged by mustard essential oil. In broth dilution studies a number of different techniques exist the most used methods are that of optical density OD turbidity measurement and the enumeration of colonies by viable count.
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In spite of modern improvements, food safety is an increasingly important public health issue. Tests of antimicrobial activity can be classified as diffusion and dilution methods. Although, they are GRAS, the application of essential oils is limited because of organoleptic changes in food.
Results antimikrobiyap that planting space had significant effect on plant diameter and very significant effect on other measured parameters except oil content, which was not significant.
Browsing by Subject “Antimikrobiyal Aktivite”
In this study the akitvite show that gram-negative E. Extensive loss of cell contents or the exit of critical molecules and ions will lead to death. H7 and Salmonella typhi was investigated. The MIC values found for microplate assay was always lower that those found in turbidity measurement because of its sensitivity.
Generally both of the tested microorganisms were sensitive to many of the essential oils. A significantly higher release of cell constituent was observed when both bacteria cells were aktigite with mustard essential oil.
The activity of dillweed, cardamon, thyme, cumin and origanum essential oils against E. Numerous investigations have confirmed the antimicrobial action of essential oils.
Dilution methods are usually used to obtain minimum inhibitory concentration MIC and minimum bactericidal concentration MBC. Therefore comparison of published data is complicated.